This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCI-VSVG
catalog :
1733
citations: 22
Reference
Zhao L, Qiu Z, Yang Z, Xu L, Pearce T, Wu Q, et al. Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolism. Nat Cancer. 2024;: pubmed publisher
Pourzia A, Olson M, Bailey S, Boroughs A, Aryal A, Ryan J, et al. Quantifying requirements for mitochondrial apoptosis in CAR T killing of cancer cells. Cell Death Dis. 2023;14:267 pubmed publisher
Rovira Clav xe9 X, Drainas A, Jiang S, Bai Y, Baron M, Zhu B, et al. Spatial epitope barcoding reveals clonal tumor patch behaviors. Cancer Cell. 2022;40:1423-1439.e11 pubmed publisher
Picarda E, Galbo P, Zong H, Rajan M, Wallenius V, Zheng D, et al. The immune checkpoint B7-H3 (CD276) regulates adipocyte progenitor metabolism and obesity development. Sci Adv. 2022;8:eabm7012 pubmed publisher
Song A, Hazlett Z, Abeykoon D, Dortch J, Dillon A, Curtiss J, et al. Branched ubiquitin chain binding and deubiquitination by UCH37 facilitate proteasome clearance of stress-induced inclusions. elife. 2021;10: pubmed publisher
Sottile R, Panjwani M, Lau C, Daniyan A, Tanaka K, Barker J, et al. Human cytomegalovirus expands a CD8+ T cell population with loss of BCL11B expression and gain of NK cell identity. Sci Immunol. 2021;6:eabe6968 pubmed publisher
de Mattos Barbosa M, Liu H, Huynh D, Shelley G, Keller E, Emmer B, et al. IgV somatic mutation of human anti-SARS-CoV-2 monoclonal antibodies governs neutralization and breadth of reactivity. JCI Insight. 2021;6: pubmed publisher
RABINOWITZ J, Sharifi H, Martin H, Marchese A, Robek M, Shi B, et al. xCT/SLC7A11 antiporter function inhibits HIV-1 infection. Virology. 2021;556:149-160 pubmed publisher
Briones B, Pisano T, Pitcher M, Haye A, Diethorn E, Engel E, et al. Adult-born granule cell mossy fibers preferentially target parvalbumin-positive interneurons surrounded by perineuronal nets. Hippocampus. 2021;: pubmed publisher
Yu L, Wang L, Kim J, Mao C, Shapiro D. Src couples estrogen receptor to the anticipatory unfolded protein response and regulates cancer cell fate under stress. Biochim Biophys Acta Mol Cell Res. 2020;:118765 pubmed publisher
Ma M, Kler S, Pan Y. Structural Neural Connectivity Analysis in Zebrafish With Restricted Anterograde Transneuronal Viral Labeling and Quantitative Brain Mapping. Front Neural Circuits. 2019;13:85 pubmed publisher
Mikeli M, Fujikawa M, Nagahisa K, Yasuda S, Yamada N, Tanabe T. Contribution of GPD2/mGPDH to an alternative respiratory chain of the mitochondrial energy metabolism and the stemness in CD133-positive HuH-7 cells. Genes Cells. 2020;25:139-148 pubmed publisher
Yu L, Wang L, Mao C, Duraki D, Kim J, Huang R, et al. Estrogen-independent Myc overexpression confers endocrine therapy resistance on breast cancer cells expressing ERαY537S and ERαD538G mutations. Cancer Lett. 2019;442:373-382 pubmed publisher
Kim Y, Um J, Yun J. A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence. J Vis Exp. 2018;: pubmed publisher
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Zhang P, Rausch C, Hastert F, Boneva B, Filatova A, Patil S, et al. Methyl-CpG binding domain protein 1 regulates localization and activity of Tet1 in a CXXC3 domain-dependent manner. Nucleic Acids Res. 2017;45:7118-7136 pubmed publisher
Weaver J, Arandjelovic S, Brown G, K Mendu S, S Schappe M, Buckley M, et al. Hematopoietic pannexin 1 function is critical for neuropathic pain. Sci Rep. 2017;7:42550 pubmed publisher
Huggins I, Brafman D, Willert K. Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells. Methods Mol Biol. 2016;1481:161-81 pubmed publisher
La Porta J, Matus Nicodemos R, Valentin Acevedo A, Covey L. The RNA-Binding Protein, Polypyrimidine Tract-Binding Protein 1 (PTBP1) Is a Key Regulator of CD4 T Cell Activation. PLoS ONE. 2016;11:e0158708 pubmed publisher
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Matus Nicodemos R, Vavassori S, Castro Faix M, Valentin Acevedo A, Singh K, Marcelli V, et al. Polypyrimidine tract-binding protein is critical for the turnover and subcellular distribution of CD40 ligand mRNA in CD4+ T cells. J Immunol. 2011;186:2164-71 pubmed publisher
product information
Catalog Number :
1733
Product Name :
pCI-VSVG
article :
doi
id92
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonemodified pUC19
backbone_mutation
backbone_origin
backbone_size5553
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This is an envelope construct and is a part of the FELIX vector system from Nolan lab. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB.
growth strain :
VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameVSV-G Envelope
shRNA_sequence
size
species
tags
resistance markers :
364
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA