This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
2xHA MED10 pBacPAK8
catalog :
17321
citations: 1
Reference
Koutelou E, Sato S, Tomomori Sato C, Florens L, Swanson S, Washburn M, et al. Neuralized-like 1 (Neurl1) targeted to the plasma membrane by N-myristoylation regulates the Notch ligand Jagged1. J Biol Chem. 2008;283:3846-53 pubmed
product information
Catalog Number :
17321
Product Name :
2xHA MED10 pBacPAK8
article :
doi10.1074/jbc.M706974200
id1917
pubmed_id18077452
bacterial resistance :
Ampicillin
cloning :
backbonepBacPAK8
backbone_mutation
backbone_originClontech
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
The Med10 sequence was inserted between XhoI and Kpn1 of pBacPAK8 polylinker after changing the original BacPAK8 BamHI site to NheI. Plasmid can be used for Med10 expression or, by cutting out and replacing Med10 CDS with something else, for construction of BacPAK8 derivatives expressing 2xHA-tagged proteins.
origin :
37
pi :
alt_names
Med10
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5XhoI
promoter
sequencing_primer_3Bac 2
sequencing_primer_5Bac 1
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC78613, AA959813, AI385605, D13Wsu50e
geneMed10
id28077
genbank_ids
mutation
nameMED10
shRNA_sequence
size500
species
10090
Mus musculus
tags
locationN terminal on backbone
tag2xHA
resistance markers :
86
87
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA