This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAX198
catalog :
173042
citations: 1
Reference
Hussmann J, Ling J, Ravisankar P, Yan J, Cirincione A, Xu A, et al. Mapping the genetic landscape of DNA double-strand break repair. Cell. 2021;184:5653-5669.e25 pubmed publisher
product information
Catalog Number :
173042
Product Name :
pAX198
article :
doi10.1016/j.cell.2021.10.002
id28219905
pubmed_id34672952
bacterial resistance :
Ampicillin
cloning :
backbonepU6-sgRNA EF1Alpha-puro-T2A-BFP
backbone_mutationInsert HBB target region
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth strain :
Custom vector for sgRNA library construction
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promotermouse U6
sequencing_primer_3CACCGGTTCAATTGCCGAC
sequencing_primer_5cagcacaaaaggaaactcacc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD113t-C, ECYT6, beta-globin
geneHBB
id3043
genbank_ids
mutation
nameHBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR
shRNA_sequencesgGFP-NT2
size
species
9606
Homo sapiens
tags
resistance markers :
5688
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA