This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE-2xFLAG-2xSTREP-CCND1(T286A)-Puro
catalog :
172633
citations: 1
Reference
Simoneschi D, Róna G, Zhou N, Jeong Y, Jiang S, Milletti G, et al. CRL4AMBRA1 is a master regulator of D-type cyclins. Nature. 2021;592:789-793 pubmed publisher
product information
Catalog Number :
172633
Product Name :
pBABE-2xFLAG-2xSTREP-CCND1(T286A)-Puro
article :
doi10.1038/s41586-021-03445-y
id28216781
pubmed_id33854235
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-Puro
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells
origin :
37
pi :
alt_names
cyclin D1
BCL1
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCL1, D11S287E, PRAD1, U21B31
geneCCND1
id595
genbank_ids
mutationThr286Ala
nameCCND1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tag2xFLAG-2xSTREP
resistance markers :
376
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA