This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter
catalog :
172597
citations: 1
Reference
Yu Y, Alvarado R, Petty L, Bohlender R, Shaw D, Below J, et al. Polygenic risk impacts PDGFRA mutation penetrance in non-syndromic cleft lip and palate. Hum Mol Genet. 2022;31:2348-2357 pubmed publisher
product information
Catalog Number :
172597
Product Name :
pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter
article :
doi10.1093/hmg/ddac037
id28216960
pubmed_id35147171
bacterial resistance :
Ampicillin
cloning :
backbonepCEP4
backbone_mutation
backbone_originInvitrogen
backbone_size10375
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
EGFP-PEST_Reporter
growth strain :
Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.
origin :
37
pi :
alt_names
Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
PDGFRA
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3pGEX 3'
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD140A, PDGFR-2, PDGFR2
genePDGFRA
id5156
genbank_ids
mutationPDGFRA-K627R
namemyc-PDGFRA-K627R
shRNA_sequence
size3324
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagExtracellular c-myc epitope tag
locationN terminal on insert
tagSignal peptide from influenza HA
plasmid copy :
Erik Procko (Addgene plasmid # 136456)
resistance markers :
5557
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA