This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET_His6-SUMO-TEV-TtCsm6
catalog :
172486
citations: 1
Reference
Liu T, Knott G, Smock D, Desmarais J, Son S, Bhuiya A, et al. Accelerated RNA detection using tandem CRISPR nucleases. Nat Chem Biol. 2021;17:982-988 pubmed publisher
product information
Catalog Number :
172486
Product Name :
pET_His6-SUMO-TEV-TtCsm6
article :
doi10.1038/s41589-021-00842-2
id28216937
pubmed_id34354262
bacterial resistance :
Kanamycin
cloning :
backbonepET
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Codon-optimized TtCsm6 gene ordered as a synthetic gene from GenScript in pUC57. It was then subcloned into a bacterial expression vector by QB3 Macrolab at UC Berkeley. Please visit https://www.medrxiv.org/content/10.1101/2021.03.19.21253328v1 for medRxiv preprint.
growth strain :
Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.
origin :
37
pi :
alt_names
TTHB152
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7 promoter and lac operator
sequencing_primer_3T7 reverse (5'-GCTAGTTATTGCTCAGCGG-3')
sequencing_primer_5SUMO forward (5'-ttattgaggctcacagagaac-3')
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesTTH_RS10780, TTHB152
genecsm6
id3169219
genbank_ids
mutation
nameT. thermophilus Csm6 (codon optimized)
shRNA_sequence
size
species
100
Synthetic
tags
locationN terminal on backbone
tagHis-SUMO-TEV
plasmid copy :
GenScript and QB3 Macrolab (UC Berkeley)
resistance markers :
1322
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA