pLV_PP1-mCitrine | Addgene 172472 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLV_PP1-mCitrine

catalog :

172472

citations: 2

Reference
Bell G, Rincón E, Akdoğan E, Collins S. Optogenetic control of receptors reveals distinct roles for actin- and Cdc42-dependent negative signals in chemotactic signal processing. Nat Commun. 2021;12:6148 pubmed publisher
Hadjitheodorou A, Bell G, Ellett F, Shastry S, Irimia D, Collins S, et al. Directional reorientation of migrating neutrophils is limited by suppression of receptor input signaling at the cell rear through myosin II activity. Nat Commun. 2021;12:6619 pubmed publisher

product information

Catalog Number :

172472

Product Name :

pLV_PP1-mCitrine

article :

doi	10.1038/s41467-021-26371-z
id	28216927
pubmed_id	34785668

bacterial resistance :

Ampicillin

cloning :

backbone

pLenti 2nd gen

backbone_mutation

backbone_origin

backbone_size

8740

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth strain :

Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

origin :

pi :

alt_names

zebrafish parapinopsina (PP1)

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	BamHI
promoter	EF1alpha
sequencing_primer_3	cgtcttttggcaatgtgagg
sequencing_primer_5	ggagtacgtcgtctttaggt
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	pp1, uvpp
gene	parapinopsina
id	100331083

genbank_ids

mutation

name

prolactinSS-PP1-mCitrine

shRNA_sequence

size

1869

species

7955

Danio rerio