This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTE1062
catalog :
172356
citations: 1
product information
Catalog Number :
172356
Product Name :
pTE1062
article :
| doi | 10.1007/978-1-4939-7309-5_10 |
| id | 28216903 |
| pubmed_id | 29130169 |
bacterial resistance :
Hygromycin
cloning :
| backbone | pSET152 | ||||
| backbone_mutation | Cloning of ScbR under native promoter from Streptomyces coelicolor chromosome. Insertion of kanamycin resistance gene under regulation of kasOp promoter (SCB regulated). Insertion of hygromycin resistance gene for selection. | ||||
| backbone_origin | |||||
| backbone_size | 5715 | ||||
| promoter | |||||
| sequencing_primer_3 | |||||
| sequencing_primer_5 | |||||
| vector_types |
|
growth notes :
Biarnes-Carrera, Marc, Chang Kwon Lee, Takuya Nihira, Rainer Breitling, and Eriko Takano. Orthogonal Regulatory Circuits for Escherichia Coli Based on the -Butyrolactone System of Streptomyces Coelicolor . ACS Synthetic Biology 7, no. 4 (2018): 1043 55. https://doi.org/10.1021/acssynbio.7b00425. pTE1062 is replicative in E. coli via pMB1 type origin, and carrying oriT (RP4) for delivery to Streptomyces by conjugation. In Streptomyces, pTE1062 is not replicative, and therefore selection is on integration into the genome mediated by phiC31 integrase.
growth strain :
Detection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.
origin :
37
pi :
| ||||||||||||||||||||||||||||||||||||||||||||
|
resistance markers :
| 5563 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments