This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1 puro Nodamura B2
catalog :
17228
citations: 4
Reference
Kashkin K, Kotova E, Alekseenko I, Bulanenkova S, Akopov S, Kopantzev E, et al. Efficient Selection of Enhancers and Promoters from MIA PaCa-2 Pancreatic Cancer Cells by ChIP-lentiMPRA. Int J Mol Sci. 2022;23: pubmed publisher
Delgado R, Allen D, Keefe M, Mancia Leon W, Ziffra R, Crouch E, et al. Individual human cortical progenitors can produce excitatory and inhibitory neurons. Nature. 2021;: pubmed publisher
Bastin D, Aitken A, Pelin A, Pikor L, Crupi M, Huh M, et al. Enhanced susceptibility of cancer cells to oncolytic rhabdo-virotherapy by expression of Nodamura virus protein B2 as a suppressor of RNA interference. J Immunother Cancer. 2018;6:62 pubmed publisher
Sullivan C, Ganem D. A virus-encoded inhibitor that blocks RNA interference in mammalian cells. J Virol. 2005;79:7371-9 pubmed publisher
product information
Catalog Number :
17228
Product Name :
pcDNA3.1 puro Nodamura B2
article :
doi10.1128/JVI.79.12.7371-7379.2005
id1895
pubmed_id15919892
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 puro+
backbone_mutation
backbone_origin
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The gene was cloned into the KpnI/XbaI sites within the pcDNA3.1 puro+ vector. The pcDNA3.1 puro+ vector was derived from the pcDNA3.1 neo+ vector by swapping the puro cassette for the Neo gene.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5KpnI
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneNoVsRNA1gp2
id962118
genbank_ids
mutation2 silent point mutations
nameNodamura B2
shRNA_sequence
size429
species
Nodamura Virus
tags
resistance markers :
510
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA