This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMXs-hOCT3/4
catalog :
17217
citations: 68
Reference
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Setthawong P, Phakdeedindan P, Techakumphu M, Tharasanit T. Molecular signature and colony morphology affect in vitro pluripotency of porcine induced pluripotent stem cells. Reprod Domest Anim. 2021;56:1104-1116 pubmed publisher
Wang L, Zhao H, Wu J, Ren J, Luo H, Zuo X, et al. An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual. Stem Cell Res. 2021;53:102336 pubmed publisher
Sanchez Vazquez R, Martinez P, Blasco M. AKT-dependent signaling of extracellular cues through telomeres impact on tumorigenesis. PLoS Genet. 2021;17:e1009410 pubmed publisher
Sanzhez Mata A, Ferez Gomez A, González Muñoz E. Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs. STAR Protoc. 2020;1:100183 pubmed publisher
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Kim K, Yoon J, Kim J, Röpke A, Shin B, Wook Han D, et al. Generation of a human iPSC line (MPIi007-A) from a patient with Metachromatic leukodystrophy. Stem Cell Res. 2020;48:101993 pubmed publisher
Kim K, Wu Y, Yoon J, Adachi K, Wu G, Velychko S, et al. Reprogramming competence of OCT factors is determined by transactivation domains. Sci Adv. 2020;6: pubmed publisher
Kim K, Yoon J, Shin B, Röpke A, Han D, Scholer H. Generation of a human iPSC line (MPIi006-A) from a patient with Pelizaeus-Merzbacher disease. Stem Cell Res. 2020;46:101839 pubmed publisher
Glaß H, Neumann P, Pal A, Reinhardt P, Storch A, Sterneckert J, et al. Combined Dendritic and Axonal Deterioration Are Responsible for Motoneuronopathy in Patient-Derived Neuronal Cell Models of Chorea-Acanthocytosis. Int J Mol Sci. 2020;21: pubmed publisher
Uysal B, Löffler H, Rosa F, Lerche H, Schwarz N. Generation of an induced pluripotent stem cell (iPSC) line (HIHDNEi003-A) from a patient with developmental and epileptic encephalopathy carrying a KCNA2 (p.Thr374Ala) mutation. Stem Cell Res. 2019;40:101543 pubmed publisher
Dutton L, Dudhia J, Guest D, Connolly D. Inducing Pluripotency in the Domestic Cat (Felis catus). Stem Cells Dev. 2019;28:1299-1309 pubmed publisher
Park J, Jun E, Son D, Hong W, Jang J, Yun W, et al. Overexpression of Nanog in amniotic fluid-derived mesenchymal stem cells accelerates dermal papilla cell activity and promotes hair follicle regeneration. Exp Mol Med. 2019;51:72 pubmed publisher
Liu L, Li Y, Guo N, Li S, Ma X, Zhang Y, et al. Therapeutic Potential of Patient iPSC-Derived iMelanocytes in Autologous Transplantation. Cell Rep. 2019;27:455-466.e5 pubmed publisher
Setthawong P, Phakdeedindan P, Tiptanavattana N, Rungarunlert S, Techakumphu M, Tharasanit T. Generation of porcine induced-pluripotent stem cells from Sertoli cells. Theriogenology. 2019;127:32-40 pubmed publisher
Schwarz N, Uysal B, Rosa F, Löffler H, Mau Holzmann U, Liebau S, et al. Generation of an induced pluripotent stem cell (iPSC) line from a patient with developmental and epileptic encephalopathy carrying a KCNA2 (p.Leu328Val) mutation. Stem Cell Res. 2018;33:6-9 pubmed publisher
Pane L, Fulcoli F, Cirino A, Altomonte A, Ferrentino R, Bilio M, et al. Tbx1 represses Mef2c gene expression and is correlated with histone 3 deacetylation of the anterior heart field enhancer. Dis Model Mech. 2018;11: pubmed publisher
Phakdeedindan P, Setthawong P, Tiptanavattana N, Rungarunlert S, Ingrungruanglert P, Israsena N, et al. Rabbit induced pluripotent stem cells retain capability of in vitro cardiac differentiation. Exp Anim. 2019;68:35-47 pubmed publisher
Sambathkumar R, Akkerman R, Dastidar S, Roelandt P, Kumar M, Bajaj M, et al. Generation of hepatocyte- and endocrine pancreatic-like cells from human induced endodermal progenitor cells. PLoS ONE. 2018;13:e0197046 pubmed publisher
Baird A, Lindsay T, Everett A, Iyemere V, Paterson Y, McClellan A, et al. Osteoblast differentiation of equine induced pluripotent stem cells. Biol Open. 2018;7: pubmed publisher
Thekkeparambil Chandrabose S, Sriram S, Subramanian S, Cheng S, Ong W, Rozen S, et al. Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming. Stem Cell Res Ther. 2018;9:68 pubmed publisher
Salas S, Ng N, Gerami Naini B, Anchan R. Induced Pluripotent Stem Cells from Ovarian Tissue. Curr Protoc Hum Genet. 2017;95:21.10.1-21.10.22 pubmed publisher
Chakritbudsabong W, Sariya L, Pamonsupornvichit S, Pronarkngver R, Chaiwattanarungruengpaisan S, Ferreira J, et al. Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D. Stem Cell Res. 2017;24:21-24 pubmed publisher
Bansho Y, Lee J, Nishida E, Nakajima Koyama M. Identification and characterization of secreted factors that are upregulated during somatic cell reprogramming. FEBS Lett. 2017;591:1584-1600 pubmed publisher
Verusingam N, Yeap S, Ky H, Paterson I, Khoo S, Cheong S, et al. Susceptibility of Human Oral Squamous Cell Carcinoma (OSCC) H103 and H376 cell lines to Retroviral OSKM mediated reprogramming. Peerj. 2017;5:e3174 pubmed publisher
Zhang L, Jambusaria A, Hong Z, Marsboom G, Toth P, Herbert B, et al. SOX17 Regulates Conversion of Human Fibroblasts Into Endothelial Cells and Erythroblasts by Dedifferentiation Into CD34+ Progenitor Cells. Circulation. 2017;135:2505-2523 pubmed publisher
Bharathan S, Manian K, Aalam S, Palani D, Deshpande P, Pratheesh M, et al. Systematic evaluation of markers used for the identification of human induced pluripotent stem cells. Biol Open. 2017;6:100-108 pubmed publisher
Zhu Z, Li C, Zeng Y, Ding J, Qu Z, Gu J, et al. PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs. Cell Stem Cell. 2017;20:274-289.e7 pubmed publisher
Rivera T, Haggblom C, Cosconati S, Karlseder J. A balance between elongation and trimming regulates telomere stability in stem cells. Nat Struct Mol Biol. 2017;24:30-39 pubmed publisher
Zapata Linares N, Rodriguez S, Mazo M, Abizanda G, Andreu E, Barajas M, et al. Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue. Stem Cell Res. 2016;16:20-3 pubmed publisher
Eid W, Abdel Rehim W. Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A. Biol Chem. 2016;397:1205-1213 pubmed publisher
Zukeran A, Takahashi A, Takaoka S, Mohamed H, Suzuki T, Ikematsu S, et al. The CCR4-NOT deadenylase activity contributes to generation of induced pluripotent stem cells. Biochem Biophys Res Commun. 2016;474:233-239 pubmed publisher
Villa Diaz L, Kim J, Laperle A, Palecek S, Krebsbach P. Inhibition of Focal Adhesion Kinase Signaling by Integrin ?6?1 Supports Human Pluripotent Stem Cell Self-Renewal. Stem Cells. 2016;34:1753-64 pubmed publisher
Sancho Martinez I, Nivet E, Xia Y, Hishida T, Aguirre A, Ocampo A, et al. Establishment of human iPSC-based models for the study and targeting of glioma initiating cells. Nat Commun. 2016;7:10743 pubmed publisher
Bhutani K, Nazor K, Williams R, Tran H, Dai H, Dzakula Z, et al. Whole-genome mutational burden analysis of three pluripotency induction methods. Nat Commun. 2016;7:10536 pubmed publisher
Malan D, Zhang M, Stallmeyer B, Müller J, Fleischmann B, Schulze Bahr E, et al. Human iPS cell model of type 3 long QT syndrome recapitulates drug-based phenotype correction. Basic Res Cardiol. 2016;111:14 pubmed publisher
Ryu J, Park H, Park J, Lee H, Rhee W, Park T. Soluble expression and stability enhancement of transcription factors using 30Kc19 cell-penetrating protein. Appl Microbiol Biotechnol. 2016;100:3523-32 pubmed publisher
Qian X, Kim J, Tong W, Villa Diaz L, Krebsbach P. DPPA5 Supports Pluripotency and Reprogramming by Regulating NANOG Turnover. Stem Cells. 2016;34:588-600 pubmed publisher
Saito Y, Nakamura K, Yoshida M, Sugiyama H, Ohe T, Kurokawa J, et al. Enhancement of Spontaneous Activity by HCN4 Overexpression in Mouse Embryonic Stem Cell-Derived Cardiomyocytes - A Possible Biological Pacemaker. PLoS ONE. 2015;10:e0138193 pubmed publisher
Gallego Romero I, Pavlovic B, Hernando Herraez I, Zhou X, WARD M, Banovich N, et al. A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics. elife. 2015;4:e07103 pubmed publisher
Doerr J, Böckenhoff A, Ewald B, Ladewig J, Eckhardt M, Gieselmann V, et al. Arylsulfatase A Overexpressing Human iPSC-derived Neural Cells Reduce CNS Sulfatide Storage in a Mouse Model of Metachromatic Leukodystrophy. Mol Ther. 2015;23:1519-31 pubmed publisher
Fuerstenau Sharp M, Zimmermann M, Stark K, Jentsch N, Klingenstein M, Drzymalski M, et al. Generation of highly purified human cardiomyocytes from peripheral blood mononuclear cell-derived induced pluripotent stem cells. PLoS ONE. 2015;10:e0126596 pubmed publisher
Chuang C, Huang M, Chen H, Tseng L, Yu C, Stone L, et al. Granulosa cell-derived induced pluripotent stem cells exhibit pro-trophoblastic differentiation potential. Stem Cell Res Ther. 2015;6:14 pubmed publisher
Tatarishvili J, Oki K, Monni E, Koch P, Memanishvili T, Buga A, et al. Human induced pluripotent stem cells improve recovery in stroke-injured aged rats. Restor Neurol Neurosci. 2014;32:547-58 pubmed publisher
Hartfield E, Yamasaki Mann M, Ribeiro Fernandes H, Vowles J, James W, Cowley S, et al. Physiological characterisation of human iPS-derived dopaminergic neurons. PLoS ONE. 2014;9:e87388 pubmed publisher
Srinivasakumar N, Zaboikin M, Tidball A, Aboud A, Neely M, Ess K, et al. Gammaretroviral vector encoding a fluorescent marker to facilitate detection of reprogrammed human fibroblasts during iPSC generation. Peerj. 2013;1:e224 pubmed publisher
Goh P, Caxaria S, Casper C, Rosales C, Warner T, Coffey P, et al. A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific induced pluripotent stem (iPS) cells. PLoS ONE. 2013;8:e81622 pubmed publisher
Nagura S, Otaka S, Koike C, Okabe M, Yoshida T, Fathy M, et al. Effect of exogenous Oct4 overexpression on cardiomyocyte differentiation of human amniotic mesenchymal cells. Cell Reprogram. 2013;15:471-80 pubmed publisher
Wezel F, Pearson J, Kirkwood L, Southgate J. Differential expression of Oct4 variants and pseudogenes in normal urothelium and urothelial cancer. Am J Pathol. 2013;183:1128-1136 pubmed publisher
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Ruiz S, Gore A, Li Z, Panopoulos A, Montserrat N, Fung H, et al. Analysis of protein-coding mutations in hiPSCs and their possible role during somatic cell reprogramming. Nat Commun. 2013;4:1382 pubmed publisher
Chan Y, Goke J, Lu X, Venkatesan N, Feng B, Su I, et al. A PRC2-dependent repressive role of PRDM14 in human embryonic stem cells and induced pluripotent stem cell reprogramming. Stem Cells. 2013;31:682-92 pubmed publisher
Mahalingam D, Kong C, Lai J, Tay L, Yang H, Wang X. Reversal of aberrant cancer methylome and transcriptome upon direct reprogramming of lung cancer cells. Sci Rep. 2012;2:592 pubmed publisher
Frank S, Zhang M, Scholer H, Greber B. Small molecule-assisted, line-independent maintenance of human pluripotent stem cells in defined conditions. PLoS ONE. 2012;7:e41958 pubmed publisher
Mak S, Huang Y, Iranmanesh S, Vangipuram M, Sundararajan R, Nguyen L, et al. Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage. Stem Cells Int. 2012;2012:140427 pubmed publisher
Sugii S, Kida Y, Berggren W, Evans R. Feeder-dependent and feeder-independent iPS cell derivation from human and mouse adipose stem cells. Nat Protoc. 2011;6:346-58 pubmed publisher
Liu G, Barkho B, Ruiz S, Diep D, Qu J, Yang S, et al. Recapitulation of premature ageing with iPSCs from Hutchinson-Gilford progeria syndrome. Nature. 2011;472:221-5 pubmed publisher
Ruiz S, Brennand K, Panopoulos A, Herrerias A, Gage F, Izpisua Belmonte J. High-efficient generation of induced pluripotent stem cells from human astrocytes. PLoS ONE. 2010;5:e15526 pubmed publisher
Spence J, Mayhew C, Rankin S, Kuhar M, Vallance J, Tolle K, et al. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature. 2011;470:105-9 pubmed publisher
Kamata M, Liu S, Liang M, Nagaoka Y, Chen I. Generation of human induced pluripotent stem cells bearing an anti-HIV transgene by a lentiviral vector carrying an internal murine leukemia virus promoter. Hum Gene Ther. 2010;21:1555-67 pubmed publisher
Germanguz I, Sedan O, Zeevi Levin N, Shtrichman R, Barak E, Ziskind A, et al. Molecular characterization and functional properties of cardiomyocytes derived from human inducible pluripotent stem cells. J Cell Mol Med. 2011;15:38-51 pubmed publisher
Hotta A, Cheung A, Farra N, Garcha K, Chang W, Pasceri P, et al. EOS lentiviral vector selection system for human induced pluripotent stem cells. Nat Protoc. 2009;4:1828-44 pubmed publisher
Moretti A, Bellin M, Jung C, Thies T, Takashima Y, Bernshausen A, et al. Mouse and human induced pluripotent stem cells as a source for multipotent Isl1+ cardiovascular progenitors. FASEB J. 2010;24:700-11 pubmed publisher
Byrne J, Nguyen H, Reijo Pera R. Enhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts. PLoS ONE. 2009;4:e7118 pubmed publisher
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007;131:861-72 pubmed
product information
Catalog Number :
17217
Product Name :
pMXs-hOCT3/4
article :
doi10.1016/j.cell.2007.11.019
id1893
pubmed_id18035408
bacterial resistance :
Ampicillin
cloning :
backbonepMXs
backbone_mutation
backbone_origin
backbone_size4600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
growth strain :
Retroviral expression of human OCT3/4
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5pMX-S1811
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
genbank_ids
mutationNone
nameOCT3/4
shRNA_sequence
size1100
species
9606
Homo sapiens
tags
plasmid copy :
pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
resistance markers :
596
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA