M71 pGL3 Sp5l P1203 mut | Addgene 17209 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

M71 pGL3 Sp5l P1203 mut

catalog :

17209

product information

Catalog Number :

17209

Product Name :

M71 pGL3 Sp5l P1203 mut

article :

doi
id	1889
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pGL3-Basic

backbone_mutation

backbone_origin

promega

backbone_size

4818

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Luciferase

growth notes :

Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon. (as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 clone. upstream of firefly luciferase in the pGL3 Basic promoter. 6 of the putative Tcf/Lef sites mutated. (all introduce additional restriction sites); cloned by PCR-based in vitro synthesis from 28 overlapping. oligos. cloned NdeI / HindIII to exchange wild-type fragment in pGL3 Sp5l P1203. Species of Sequence Origin: zebrafish.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	LucNRev
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Spr2, YF-335, cb577, fc23b01, s, sb:cb57, sb:cb577, wu:fc23b01
gene	sp5l
id	324261

genbank_ids

mutation

6 of the putative Tcf/Lef sites mutated

name

Sp5l promoter

shRNA_sequence

size

1250

species

7955

Danio rerio