This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
M70 pGL3 Sp5l P1203
catalog :
17208
product information
Catalog Number :
17208
Product Name :
M70 pGL3 Sp5l P1203
article :
doi
id1889
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepGL3-Basic
backbone_mutation
backbone_originpromega
backbone_size4818
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Luciferase
growth notes :
Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon (as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 cloned upstream of firefly luciferase in the pGL3 Basic promoter. For testing whether this fragment is Wnt responsive. Amplified from KWT genomic DNA using oligos 328 & 329, cloned XhoI / HindIII in pGL3-Basic (from Ajamete). Sequenced using 328 & 329 (ergo junctions not sequenced); 2 SNPs to Ensembl sequence.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5XhoI
promoter
sequencing_primer_3LucNRev
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSpr2, YF-335, cb577, fc23b01, s, sb:cb57, sb:cb577, wu:fc23b01
genesp5l
id324261
genbank_ids
mutation
nameSp5l promoter
shRNA_sequence
size1250
species
7955
Danio rerio
tags
locationC terminal on backbone
tagluciferase
resistance markers :
348
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA