M29 NacMS+LEF>Nac | Addgene 17179 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

M29 NacMS+LEF>Nac

catalog :

17179

product information

Catalog Number :

17179

Product Name :

M29 NacMS+LEF>Nac

article :

doi
id	1889
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone	n/a
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth notes :

Note for Use: Description: The NacMS>Nac construct (with mutations in endogenous. LEF sites) was engineered to have 5 LEF sites added at a new position to. rescue beta-catenin responsiveness of the promoter. Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.

origin :

pi :

alt_names

nacre

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	nacre, z3A.1
gene	mitfa
id	30080

genbank_ids

AF211890

mutation

All three Tcf/Lef sites are mutated, but 5 LEF sites are added at a new position

name

nacre promoter driving nacre cDNA

shRNA_sequence

size

species

7955

Danio rerio