This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Str-KDEL_TNF-SBP-RpHLuorin2
catalog :
171722
citations: 1
Reference
Linders P, Ioannidis M, ter Beest M, van den Bogaart G. Fluorescence Lifetime Imaging of pH along the Secretory Pathway. ACS Chem Biol. 2022;17:240-251 pubmed publisher
product information
Catalog Number :
171722
Product Name :
Str-KDEL_TNF-SBP-RpHLuorin2
article :
doi10.1021/acschembio.1c00907
id28216762
pubmed_id35000377
bacterial resistance :
Ampicillin
cloning :
backbonepIRESneo3
backbone_mutationRUSH TNF -RpHLuorin2 was generated by replacing EGFP from Str-KDEL_TNF-SBP-EGFP (Addgene plasmid #65278) with synthetic RpHLuorin2 (Genscript) with restriction sites SbfI and BsrGI.
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043
growth strain :
Synchronize trafficking of TNFa-RpHLuorin2
origin :
37
pi :
alt_names
DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F
cloning
clone_methodRestriction Enzyme
cloning_site_3BsrGI
cloning_site_5SbfI
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5IRES-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDIF, TNF-alpha, TNFA, TNFSF2, TNLG1F
geneTNF
id7124
genbank_ids
NM_000594.3
mutation
nameTNF
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagratiometric pHluorin2
locationC terminal on insert
tagStreptavidin Binding Protein (SBP)
plasmid copy :
TNF -RpHLuorin2 was generated by replacing EGFP from Str-KDEL_TNF-SBP-EGFP (Addgene plasmid #65278) with synthetic RpHLuorin2 (Genscript)
resistance markers :
3431
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA