LAMP1-RpHLuorin2 | Addgene 171720 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

LAMP1-RpHLuorin2

catalog :

171720

citations: 1

Reference
Linders P, Ioannidis M, ter Beest M, van den Bogaart G. Fluorescence Lifetime Imaging of pH along the Secretory Pathway. ACS Chem Biol. 2022;17:240-251 pubmed publisher

product information

Catalog Number :

171720

Product Name :

LAMP1-RpHLuorin2

article :

doi	10.1021/acschembio.1c00907
id	28216762
pubmed_id	35000377

bacterial resistance :

Kanamycin

cloning :

backbone

pRpHluorin-N1

backbone_mutation

backbone_origin

Clontech

backbone_size

4730

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Targeting to LAMP1-positive compartments was achieved by inserting the signal sequence of LAMP1 (LAMP1-mGFP74, Addgene plasmid #34831) N-terminal to RpHLuorin2 with restriction sites HindIII and AgeI. Then, the luminal domain of LAMP1 was placed C-terminal to RpHLuorin2 after a flexible GSGS linker with restriction sites BsrGI and NotI. Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043

growth strain :

Lysosomal expression of ratiometric pHLuorin2

origin :

pi :

alt_names

CD107a, LAMPA, LGP120

cloning

clone_method	Restriction Enzyme
cloning_site_3	AgeI
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3	GTCCAGCTCGACCAGGATGGG
sequencing_primer_5	TAACAACTCCGCCCCATT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CD107a, LAMPA, LGP120
gene	LAMP1
id	3916

genbank_ids

NM_005561.3

mutation

name

Ratiometric pHLuorin2 fused to LAMP1

shRNA_sequence

size

species

9606

Homo sapiens