This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GalT-RpHLuorin2
catalog :
171719
citations: 1
Reference
Linders P, Ioannidis M, ter Beest M, van den Bogaart G. Fluorescence Lifetime Imaging of pH along the Secretory Pathway. ACS Chem Biol. 2022;17:240-251 pubmed publisher
product information
Catalog Number :
171719
Product Name :
GalT-RpHLuorin2
article :
doi10.1021/acschembio.1c00907
id28216762
pubmed_id35000377
bacterial resistance :
Kanamycin
cloning :
backbonepRpHluorin-N1
backbone_mutationThe starting codon of RpHLuorin2 was removed and a flexible GGSGGS linker was added between the two protein fragments.
backbone_originClontech
backbone_size4730
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Ratiometric pHLuorin2 sequence synthesized by Genscript, original sequence from https://www.scirp.org/journal/paperinformation.aspx?paperid=5043
growth strain :
Trans-Golgi expression of ratiometric pHLuorin2
origin :
37
pi :
alt_names
B4GAL-T1, CDG2D, GGTB2, GT1, GTB, beta4Gal-T1, GalT
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5SmaI
promoterCMV
sequencing_primer_3GTCCAGCTCGACCAGGATGGG
sequencing_primer_5TAACAACTCCGCCCCATT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB4GAL-T1, CDG2D, GGTB2, GT1, GTB, beta4Gal-T1
geneB4GALT1
id2683
genbank_ids
NM_001497.3
mutation
nameRatiometric pHLuorin2 fused to B4GALT1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagratiometric pHluorin2
plasmid copy :
Truncated GalT was cloned from Addgene plasmid #87325
resistance markers :
3431
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA