-226bp goosecoid luciferase | Addgene 17158 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

-226bp goosecoid luciferase

catalog :

17158

citations: 1

Reference
Watabe T, Kim S, Candia A, Rothbächer U, Hashimoto C, Inoue K, et al. Molecular mechanisms of Spemann's organizer formation: conserved growth factor synergy between Xenopus and mouse. Genes Dev. 1995;9:3038-50 pubmed

product information

Catalog Number :

17158

Product Name :

-226bp goosecoid luciferase

article :

doi	10.1101/gad.9.24.3038
id	2151
pubmed_id	8543150

bacterial resistance :

Ampicillin

cloning :

backbone

pOLuc

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Luciferase

growth notes :

Generated by PCR using the 3' primer (nucleotides - 5 to + 12 relative to the transcription start site) and 5' primer to -226. The 3' primer contained a HindIII restriction site at its 5' terminus and the 5' primer contained a BamHI restriction site at its 5' terminus for directional cloning into pOLuc.

origin :

pi :

alt_names

goosecoid

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	BamHI
promoter
sequencing_primer_3	LucNRev
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Xgsc, goosecoid
gene	gsc-a
id	397752

genbank_ids

mutation

name

goosecoid promoter

shRNA_sequence

size

230

species

8355

Xenopus laevis