This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
M20 siamois-luciferase p01234
catalog :
17157
product information
Catalog Number :
17157
Product Name :
M20 siamois-luciferase p01234
article :
| doi | |
| id | 1889 |
| pubmed_id |
bacterial resistance :
Ampicillin
cloning :
| backbone | pGL3-Basic | |
| backbone_mutation | ||
| backbone_origin | promega | |
| backbone_size | 4818 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Plasmid History: Constructed by David Kimelman, 6/6/96 XVII p. 98. Notes for Use: The -3.0kb-siamois promoter was directionally cloned into the KpnI and BglII sites of the luciferase reporter vector pGL3B (Promega) upstream of the luciferase ATG. The -0.8kb promoter construct was generated by digesting the -3.0kb/pGL3B construct with KpnI and at an internal EcoRI site to remove 2.2 kb of intervening sequence, filling in with DNA polymerase I large fragment and religating. The Kpn site was lost but the R1 site was regenerated in the ligation. The size of the insert is approximate. Citation: Brannon et al., Gene and Development 11:2359-2370, 1997.
origin :
37
pi :
|
resistance markers :
| 348 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments