M19 pCS-twhh-dsl-1myc | Addgene 17156 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

M19 pCS-twhh-dsl-1myc

catalog :

17156

product information

Catalog Number :

17156

Product Name :

M19 pCS-twhh-dsl-1myc

article :

doi
id	1889
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone	pCS2+
backbone_mutation
backbone_origin
backbone_size	4100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth notes :

Plasmid History: To place dsl-1myc after the twhh promoter, the DNA insert of dsl-1myc was first subcloned into the EcoRI site of expression vector pCS2+, which has a cytomegalovirus promoter and a polyadenylation site. The resulting plasmids were named pCS2+-dsl-1myc. To link dsl-1myc to the twhh promoter, the dsl-1myc insert was released from pCS2+-dsl-1myc by BamHI and XhoI digestion and then subcloned into pCS-twhh-beta -gal-vec BamHI and XhoI sites by replacing the beta -gal sequence. The final construct, pCS-twhh-dsl-1myc, contains the 5.2-kb twhh promoter and dsl-1myc. Notes for Use: Target dorsalin-1 expression in zebrafish notochord. Citation: Du et al., 1997, J. Cell Biology.

origin :

pi :

alt_names

dsl-1

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	T3
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DSL1
gene	GDF2
id	396412

genbank_ids

mutation

name

dorsalin-1

shRNA_sequence

size

species

9031

Gallus gallus