This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
M18 pCS-twhh-nBeta gal Vec
catalog :
17155
product information
Catalog Number :
17155
Product Name :
M18 pCS-twhh-nBeta gal Vec
article :
doi
id1889
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepCS-NBeta gal
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
growth notes :
Parent vector: pCS-twhh-nBeta gal. Insert: twhh promoter (5.2 Kb), nBeta gal (3 Kb) Plasmid History: Source: zebrafish. Notes for Use: To make the twhh promoter into a convenient expression vector for expressing heterologous cDNAs, the BamHI site upstream of the twhh promoter in the plasmid pCS-twhh-beta -gal was deleted. The resulting plasmid, which retains the BamHI site between the promoter and the beta -gal, was isolated and designated pCS-twhh-beta -gal-vec. Genes of interest can be cloned into the BamHI and XhoI sites of this vector by replacing the beta -gal sequence. Citation: Du et al., 1997, J. Cell Biology.
origin :
37
pi :
alt_names
tiggy-winkle hedgehog
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5NA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasestwh, twhh
geneshhb
id30444
genbank_ids
mutation
nametwhh promoter
shRNA_sequence
size5200
species
7955
Danio rerio
tags
locationC terminal on backbone
tagnuclear beta-gal
resistance markers :
348
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA