This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
M16 pCS-twhh-bGFP
catalog :
17153
product information
Catalog Number :
17153
Product Name :
M16 pCS-twhh-bGFP
article :
doi
id1889
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepCS
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
growth notes :
Insert: twhh promoter (5.2 Kb), bGFP (1 Kb) Plasmid History: The reporter gene, bright green fluorescent protein (bGFP) with a serine 65 to threonine mutation (Heim et al., 1995), was cloned into vector pCS-twhh-beta -gal-vec BamHI/XhoI sites by blunt end ligation. The resulting plasmid was named pCS-twhh-bGFP. Notes for Use: Gives notochord-specific expression of GFP. Citation: Du et al., 1997, J. Cell Biology.
origin :
37
pi :
alt_names
tiggy-winkle hedgehog
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5NA
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasestwh, twhh
geneshhb
id30444
genbank_ids
mutation
nametwhh promoter
shRNA_sequence
size5200
species
7955
Danio rerio
tags
locationC terminal on backbone
tagbGFP
resistance markers :
348
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA