This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEMHE-GST-KASH5-DN
catalog :
171493
citations: 1
Reference
Cavazza T, Takeda Y, Politi A, Aushev M, Aldag P, Baker C, et al. Parental genome unification is highly error-prone in mammalian embryos. Cell. 2021;184:2860-2877.e22 pubmed publisher
product information
Catalog Number :
171493
Product Name :
pGEMHE-GST-KASH5-DN
article :
doi10.1016/j.cell.2021.04.013
id28216708
pubmed_id33964210
bacterial resistance :
Ampicillin
cloning :
backbonepGEHME
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
pGEHME
growth notes :
Dominant negative version of bovine KASH5 (amino acids 482-524) that blocks the function of the LINC complex.
growth strain :
T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA
origin :
37
pi :
alt_names
CCDC155
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterT7
sequencing_primer_3GGCTACATTTTGGGGGACAACATTTTGTAAAGT
sequencing_primer_5M13: GTAAAACGACGGCCAGT
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneCCDC155
id617651
genbank_ids
mutation
nameKASH5-DN
shRNA_sequence
size
species
32644
Other
B. tarus (bovine)
tags
locationN terminal on insert
tagGST
resistance markers :
3776
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA