This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
M10 NCAM Promoter
catalog :
17147
product information
Catalog Number :
17147
Product Name :
M10 NCAM Promoter
article :
doi
id1889
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript KS(+)
backbone_mutation
backbone_originStratagene
backbone_size3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Xenopus expression
growth notes :
Plasmid History: Constructed Paul A. Kreig, July. Note for Use: This construction contains a 1.3 Kb Dra I fragment of the NCAM promoter and should be okay for general neural expression. The fragment is cloned into the Sma I site of Bluescript KS+. The NCAM promoter does not contain a TATA box and transcription starts at numerous sites over about 350 bases. All of the potential start sites are contained in this 1.3 Kb fragment, which ends in the 5' UTR of the NCAM gene. The Dra I fragment starts at the very beginning of the GenBank sequence (starting with aaa) and ends at base 1358 of the GenBank sequence.
origin :
37
pi :
alt_names
NCAM
cloning
clone_methodRestriction Enzyme
cloning_site_3SmaI/DraI
cloning_site_5SmaI/DraI
promoter
sequencing_primer_3T7
sequencing_primer_5T3
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesncam, n-cam, ncam1
genencam1-b
id397762
genbank_ids
mutation
nameNCAM promoter
shRNA_sequence
size1300
species
8355
Xenopus laevis
tags
resistance markers :
348
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA