This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Flag-M4-AR-W741C-S81A-P82G
catalog :
171251
product information
Catalog Number :
171251
Product Name :
Flag-M4-AR-W741C-S81A-P82G
article :
doi
id28216688
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonep3XFLAG-CMV-10
backbone_mutation
backbone_originSigma-Aldrich
backbone_size6400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Flag-tagged human AR Ser-Pro mutant that binds to bicalutamide, and the exact amino position and composition may slightly vary based on human AR versions.Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
growth strain :
Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82G)
origin :
37
pi :
alt_names
Flag-AR-W741C-S81A-P82G
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV_fwd_primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
geneAR
id367
genbank_ids
mutationAR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82G)
name 3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Gly mutant
shRNA_sequence
size3000
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFlag
resistance markers :
5499
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA