This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Flag-M4-AR-S81A
catalog :
171241
citations: 1
Reference
Chen S, Gulla S, Cai C, Balk S. Androgen receptor serine 81 phosphorylation mediates chromatin binding and transcriptional activation. J Biol Chem. 2012;287:8571-83 pubmed publisher
product information
Catalog Number :
171241
Product Name :
Flag-M4-AR-S81A
article :
doi10.1074/jbc.M111.325290
id28216691
pubmed_id22275373
bacterial resistance :
Ampicillin
cloning :
backbonep3XFLAG-CMV-10
backbone_mutation
backbone_originSigma-Aldrich
backbone_size6400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Mutation in Flag-tagged AR Ser-Pro phosphorylation site, and the exact amino acid residue position and composition may slightly vary based on human AR versions. Two XmaI sites on the vector backbone and one EcoRI site at the 3'UTR of AR cDNA were deleted by DNA mutagenesis.
growth strain :
Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)
origin :
37
pi :
alt_names
Flag-AR-S81A
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV_fwd_primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
geneAR
id367
genbank_ids
mutationAR-Ser81Ala (AR-S81A)
name3xFlag-tagged human androgen receptor Ser81Ala mutant
shRNA_sequence
size3000
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFlag
resistance markers :
5499
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA