This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
LV-AR-KKKAAA
catalog :
171219
citations: 1
product information
Catalog Number :
171219
Product Name :
LV-AR-KKKAAA
article :
| doi | 10.1038/s41467-021-27077-y |
| id | 28227623 |
| pubmed_id | 34911936 |
bacterial resistance :
Ampicillin
cloning :
| backbone | Lenti-viral-Hygro | ||
| backbone_mutation | |||
| backbone_origin | |||
| backbone_size | 10000 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
The mutations occur on the nuclear localization signal (NLS) KLKK motif at the boundary between AR DBD and hinge domain. The mutant is defective in both nuclear import and transaction, and completely loses importin-alpha binding. Human androgen receptor reference: https://pubmed.ncbi.nlm.nih.gov/18319300/ Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.
growth strain :
Lenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.
origin :
37
pi :
|
resistance markers :
| 5499 |
tags :
High Copy
terms :
| Hygromycin |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments