LV-AR-AAFF | Addgene 171218 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

LV-AR-AAFF

catalog :

171218

citations: 1

Reference
Guo H, Wu Y, Nouri M, Spisak S, Russo J, Sowalsky A, et al. Androgen receptor and MYC equilibration centralizes on developmental super-enhancer. Nat Commun. 2021;12:7308 pubmed publisher

product information

Catalog Number :

171218

Product Name :

LV-AR-AAFF

article :

doi	10.1038/s41467-021-27077-y
id	28227623
pubmed_id	34911936

bacterial resistance :

Ampicillin

cloning :

backbone

Lenti-viral-Hygro

backbone_mutation

backbone_origin

backbone_size

10000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The mutations occur on the androgen receptor KVFF motif in the P-box of the zinc finger 1 that make contact with DNA. AR mutants of the KVFF motif would lose activity to activate AR-mediated transcription. Please Note: This plasmid contains a 6x Gln deletion in the 23x Gln track and a 8x Gly deletion in the 23x Gly track in androgen receptor compared to the depositor's provided sequence. These differences are not known to affect the function of the protein.

growth strain :

Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.

origin :

pi :

alt_names

AR-K580A-V581A

AR P-box mutant

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	EF-1a
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
gene	AR
id	367

genbank_ids

mutation

AR-K580A-V581A

name

AR-AAFF

shRNA_sequence

size

3000

species

9606

Homo sapiens