This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDH-EF1-TfR20-SNAP-IRES-Puro
catalog :
171018
citations: 1
Reference
FitzGerald L, Aurelio L, Chen M, Yuen D, Rennick J, Graham B, et al. A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells. Nat Commun. 2020;11:4482 pubmed publisher
product information
Catalog Number :
171018
Product Name :
pCDH-EF1-TfR20-SNAP-IRES-Puro
article :
doi10.1038/s41467-020-18082-8
id28216639
pubmed_id32901011
bacterial resistance :
Ampicillin
cloning :
backbonepCDH
backbone_mutation
backbone_originSystem Biosciences
backbone_size7659
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2020.08.20.258350v1 for bioRxiv preprint.
growth strain :
Lentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cells
origin :
37
pi :
alt_names
NM_003234
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterEF1
sequencing_primer_3gcttcggccagtaacgtta
sequencing_primer_5CAGCCGGCTCTCCACGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90
geneTFRC
id7037
genbank_ids
mutation
nameTransferrin receptor (Human) - SNAP-tag
shRNA_sequence
size2886
species
9606
Homo sapiens
tags
locationC terminal on insert
tagSNAPtag
locationN terminal on insert
tagTfR
plasmid copy :
mCherry-TFR-20 was a gift from Michael Davidson (Addgene plasmid # 55144 ; http://n2t.net/addgene:55144 ; RRID:Addgene_55144)
resistance markers :
4468
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA