This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONRp5-p2 SspB-mCherry-CShroom3
catalog :
170977
citations: 1
Reference
Mart xed nez Ara G, Taberner N, Takayama M, Sandaltzopoulou E, Villava C, Bosch Padr xf3 s M, et al. Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues. Nat Commun. 2022;13:5400 pubmed publisher
product information
Catalog Number :
170977
Product Name :
pDONRp5-p2 SspB-mCherry-CShroom3
article :
doi10.1038/s41467-022-33115-0
id28216632
pubmed_id36104355
bacterial resistance :
Kanamycin
cloning :
backbonepDONR221 p5-p2
backbone_mutation
backbone_originInvitrogen
backbone_size2597
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway cloning
growth notes :
Please visit https://doi.org/10.1101/2021.04.20.440475 for bioRxiv preprint.
growth strain :
C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13 rev: GTCATAGCTGTTTCCTG
sequencing_primer_5M13 fwd: GTAAAACGACGGCCAGT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAL022960, D5Ertd287, D5Ertd287e, Sh, Shr, Shrm, Shrm3
geneShroom3
id27428
genbank_ids
XP_006535031.1
mutationIsoform X2, deleted aminoacids 1 - 1388
nameCShroom3
shRNA_sequence
size1254
species
10090
Mus musculus
tags
locationN terminal on backbone
tagSspB
locationN terminal on insert
tagmCherry
plasmid copy :
Original mShroom3 sequence was a gift from T. Nishimura, from M.Takeichi lab. SspB sequence was a gift from H. Janovjak lab.
resistance markers :
5491
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA