This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pX458_sgAgo2_Nterm
catalog :
170920
citations: 1
Reference
Müller M, Fäh T, Schaefer M, Hermes V, Luitz J, Stalder P, et al. AGO1 regulates pericentromeric regions in mouse embryonic stem cells. Life Sci Alliance. 2022;5: pubmed publisher
product information
Catalog Number :
170920
Product Name :
pX458_sgAgo2_Nterm
article :
doi10.26508/lsa.202101277
id28225091
pubmed_id35236760
bacterial resistance :
Ampicillin
cloning :
backbonepX458
backbone_mutation
backbone_originFeng Zhang Lab (Addgene# 48138)
backbone_size9272
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
Expresses spCas9 and sgRNA targeting the N-terminus of Ago2 for N-terminal tagging
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterhU6
sequencing_primer_3
sequencing_primer_5hU6: GAGGGCCTATTTCCCATGATT
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliases1110029L17Rik, 2310051F07Rik, Eif2c2, Gerp95, Gm10365, mKIAA4215
geneAgo2
id239528
genbank_ids
mutation
namesgAgo2
shRNA_sequenceCGGCAACGCCACCATGTACT
size20
species
10090
Mus musculus
tags
resistance markers :
2514
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA