pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR | Addgene 170808 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

catalog :

170808

citations: 1

Reference
Wang S, Matsumoto K, Lish S, Cartagena Rivera A, Yamada K. Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion. Cell. 2021;184:3702-3716.e30 pubmed publisher

product information

Catalog Number :

170808

Product Name :

pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

article :

doi	10.1016/j.cell.2021.05.015
id	28216665
pubmed_id	34133940

bacterial resistance :

Ampicillin

cloning :

backbone

pSLQ2842 (Addgene #84244)

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint.

growth strain :

PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	Unknown
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

Synonymous mutation in PYL1 to remove XhoI site (inherited from pSLQ2842; Addgene #84244)

name

GID1-VPR-IRES-HygR

shRNA_sequence

size

4281

species

3702

Arabidopsis thaliana

100

Synthetic

32644

Other

E. coli

tags