This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ZE38 pCS2P+ sp5l ORF
catalog :
17079
product information
Catalog Number :
17079
Product Name :
ZE38 pCS2P+ sp5l ORF
article :
doi
id1885
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepCS2P+ (modified pCS2+)
backbone_mutation
backbone_origin
backbone_size4100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Zebrafish Expression
growth notes :
Description: zebrafish sp5-like (sp5l) zinc-finger transcription factor of the SP5 family (cDNA clone 27i2 from the Wnt8 upregulated microarray screen) ORF only in pCS2P+. PCR amplified using primers 96 &97 from 27-i2 (pSPORT) clone and cloned EcoRI/XbaI into pCS2P+. Injection of high doses (>260 ng/ l corresponding to 200ng/ l #53.GFP) results in loss of eyes. Species of Sequence Origin: zebrafish. Notes for Use: For functional synthetic RNA, linearize with NotI, SP6 (1385bp RNA). Select clones with amp. Reference: Development, Current Biology papers from the lab (2005).
origin :
37
pi :
alt_names
sp5-like
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSpr2, YF-335, cb577, fc23b01, s, sb:cb57, sb:cb577, wu:fc23b01
genesp5l
id324261
genbank_ids
mutation
namesp5l ORF
shRNA_sequence
size1100
species
7955
Danio rerio
tags
resistance markers :
348
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA