This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ZE36 Nlk1(C387Y)-pCS2+
catalog :
17077
product information
Catalog Number :
17077
Product Name :
ZE36 Nlk1(C387Y)-pCS2+
article :
doi
id1885
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_origin
backbone_size4100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Zebrafish Expression
growth notes :
Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert. Clone is an inactive mutant of NLK1, analogous to C425Y mutant of mouse NLK which does not bind TCFs. Species of Sequence Origin: zebrafish. Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp. Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
origin :
37
pi :
alt_names
nlk
nemo-like kinase
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesnlk, nlkb, zgc:111875
genenlk1
id405892
genbank_ids
AY562552
mutationC387Y. Inactive mutant of NLK1.
nameNlk1
shRNA_sequence
size1425
species
7955
Danio rerio
tags
resistance markers :
348
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA