This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ZE33 Prickle-1-GFPnopren-pCS2+
catalog :
17074
product information
Catalog Number :
17074
Product Name :
ZE33 Prickle-1-GFPnopren-pCS2+
article :
doi
id1885
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backboneCS2+GFP-BB
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Zebrafish Expression
growth notes :
Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP. The C-terminal prenylation motif KNCIIS was deleted by PCR. Species of Sequence Origin: zebrafish. Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp. Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).
origin :
37
pi :
alt_names
pk1
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5NA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesfk71b09, pk1, prickle1, wu:fk71b09
geneprickle1a
id368249
genbank_ids
mutationPk1GFPnopren deletes the C-terminal six amino acids containing the putative prenylation site.
namePrickle-1
shRNA_sequence
size
species
7955
Danio rerio
tags
locationN terminal on backbone
tagGFP
resistance markers :
348
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA