This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONR207-MUM2sp-ADPG2
catalog :
170723
citations: 1
Reference
McGee R, Dean G, Wu D, Zhang Y, Mansfield S, Haughn G. Pectin Modification in Seed Coat Mucilage by In Vivo Expression of Rhamnogalacturonan-I- and Homogalacturonan-Degrading Enzymes. Plant Cell Physiol. 2021;: pubmed publisher
product information
Catalog Number :
170723
Product Name :
pDONR207-MUM2sp-ADPG2
article :
doi10.1093/pcp/pcab077
id28216584
pubmed_id34059917
bacterial resistance :
Gentamicin
cloning :
backbonepDONR207
backbone_mutation
backbone_originInvitrogen
backbone_size5585
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Donor vector / entry clone
growth notes :
For use with Three-way Multi-site Gateway Cloning (Invitrogen).
growth strain :
Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)
growth temp :
For selection use 7 ug/ml Gentamicin
origin :
37
pi :
alt_names
ADPG2
At2g41850
Q8RY29 (Uniprot)
cloning
clone_methodRestriction Enzyme
cloning_site_3AatII
cloning_site_5SpeI
promoter
sequencing_primer_3GTAACATCAGAGATTTTGAGACA
sequencing_primer_5TAACGCTAGCATGGATCTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT2G41850, ADPG2, ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2, T11A7.5, T11A7_5, polygalacturonase abscission zone A. thaliana
genePGAZAT
id818785
genbank_ids
AC002339
mutation
nameARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
resistance markers :
4580
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA