tetO-IRX5 | Addgene 170693 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

tetO-IRX5

catalog :

170693

citations: 1

Reference
Romero Tejeda M, Fonoudi H, Weddle C, DeKeyser J, Lenny B, Fetterman K, et al. A novel transcription factor combination for direct reprogramming to a spontaneously contracting human cardiomyocyte-like state. J Mol Cell Cardiol. 2023;182:30-43 pubmed publisher

product information

Catalog Number :

170693

Product Name :

tetO-IRX5

article :

doi	10.1016/j.yjmcc.2023.06.005
id	28216581
pubmed_id	37421991

bacterial resistance :

Ampicillin

cloning :

backbone

FU-tetO-Gateway

backbone_mutation

IRX5 ORF added to plasmid using Gateway cloning

backbone_origin

addgene plasmid #43914

backbone_size

8600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

Other

Doxycycline Inducible

growth notes :

This plasmid was generated using Gateway cloning. attB sites were added to ID1 via PCR reaction using pcDNA3.1-C-(k)DYK-IRX5 (Genscript) as a template. Resulting fragements were recombined with pDONR221 to generate an entry clone. LR reaction was performed using the destination vector FU-tetO-Gateway (Addgene Plasmid #19778).

growth strain :

doxycycline-inducible overexpression of IRX5

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	TRE promoter, Tet-ON
sequencing_primer_3
sequencing_primer_5	LNCX; T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HMMS, IRX-2a, IRXB2
gene	IRX5
id	10265

genbank_ids

NM_005853.6

mutation

name

IRX5

shRNA_sequence

size

species

9606

Homo sapiens