This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
tetO-ETS2
catalog :
170685
citations: 1
Reference
Romero Tejeda M, Fonoudi H, Weddle C, DeKeyser J, Lenny B, Fetterman K, et al. A novel transcription factor combination for direct reprogramming to a spontaneously contracting human cardiomyocyte-like state. J Mol Cell Cardiol. 2023;182:30-43 pubmed publisher
product information
Catalog Number :
170685
Product Name :
tetO-ETS2
article :
doi10.1016/j.yjmcc.2023.06.005
id28216581
pubmed_id37421991
bacterial resistance :
Ampicillin
cloning :
backboneFU-tetO-Gateway
backbone_mutationETS2 ORF added to plasmid using Gateway cloning
backbone_originaddgene plasmid #43914
backbone_size8600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Other
Doxycycline Inducible
growth notes :
This plasmid was generated using Gateway cloning. attB sites were added to ETS2 via PCR reaction. Resulting fragements were recombined with pDONR221 to generate an entry clone. LR reaction was performed using the destination vector FU-tetO-Gateway (Addgene Plasmid #19778).
growth strain :
doxycycline-inducible overexpression of ETS2
origin :
30
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterTRE promoter, Tet-ON
sequencing_primer_3
sequencing_primer_5LNCX; T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesETS2IT1
geneETS2
id2114
genbank_ids
mutation
nameETS2
shRNA_sequence
size1410
species
9606
Homo sapiens
tags
plasmid copy :
ETS2 cDNA was obtained from Pumin Zhang (Addgene plasmid # 28128 ; http://n2t.net/addgene:28128 ; RRID:Addgene_28128)
resistance markers :
4285
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA