ZE10 sonic hedgehog pT7TS | Addgene 17059 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

ZE10 sonic hedgehog pT7TS

catalog :

17059

product information

Catalog Number :

17059

Product Name :

ZE10 sonic hedgehog pT7TS

article :

doi
id	1885
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone	pT7TS
backbone_mutation
backbone_origin
backbone_size	3000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth notes :

Steve Ekker clone E, in vector pT7TS. Linearize with BamH1 and transcribe with T7 for sense RNA for injections. Citation: Ekker et al. (1995), Current Biology 5:944-955. Made by insertion of shh open-reading frame into the SpeI site of the pT7TS vector. The ORF was made by PCR using the following primers: shhA (5 -GGACTAG-TCACCATGCGGCTTTTGACGAGA-3 ) and shhB (5 -AGCATACTAGTAGTCTCAGCTTGAGTTTACTGA-3 ).

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SpeI
cloning_site_5	SpeI
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ShhNC, fc83d08, shh, syu, vhh-1, vhh1, wu:fc83d08
gene	shha
id	30269

genbank_ids

mutation

name

sonic hedgehog

shRNA_sequence

size

species

7955

Danio rerio