This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSynapsin_NLS_GFP_P2A_His6_NR1.
catalog :
170457
citations: 1
Reference
Li S, Raychaudhuri S, Lee S, Brockmann M, Wang J, Kusick G, et al. Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses. Nat Commun. 2021;12:677 pubmed publisher
product information
Catalog Number :
170457
Product Name :
pSynapsin_NLS_GFP_P2A_His6_NR1.
article :
doi10.1038/s41467-021-21004-x
id28216543
pubmed_id33514725
bacterial resistance :
Ampicillin
cloning :
backbonepFUGW
backbone_mutationUBC promoter replaced with neuron specific synapsin promoter. Introduced NLS-GFP for checking transfection efficiency.
backbone_origin
backbone_size9370
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expresses histidine tagged NR1 in neuronal cells
origin :
37
pi :
alt_names
NMDA receptor subunit 1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterSynapsin
sequencing_primer_3
sequencing_primer_5AGGGCATCGACTTCAAGGAGGA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGluN1, NMDAR1, NR1
geneGrin1
id24408
genbank_ids
mutation
nameNR1
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagHexa-histidin
plasmid copy :
NR1 was taken from Addgene 23999
resistance markers :
5474
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA