This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLV.EF1 .mEmerald.CD9.miR9T
catalog :
170452
citations: 1
Reference
Clayton K, Delpech J, Herron S, Iwahara N, Ericsson M, Saito T, et al. Plaque associated microglia hyper-secrete extracellular vesicles and accelerate tau propagation in a humanized APP mouse model. Mol Neurodegener. 2021;16:18 pubmed publisher
product information
Catalog Number :
170452
Product Name :
pLV.EF1 .mEmerald.CD9.miR9T
article :
doi10.1186/s13024-021-00440-9
id28216541
pubmed_id33752701
bacterial resistance :
Ampicillin
cloning :
backbonepLV
backbone_mutation
backbone_origin
backbone_size6681
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
Lentiviral plasmid that encodes mEmerald-CD9 fusion protein with microRNA 9 tandem cassette to restrict expression to microglia with EF1-alpha promoter.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5PspXI
promoterEF1a
sequencing_primer_3gaattctgcagtcgacaatcaacc
sequencing_primer_5cctttcgtcttcactcgaggtgcccgtca
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29
geneCD9
id928
genbank_ids
NM_001769.4
NM_001330312.2
mutation
nameCD9
shRNA_sequence
size1431
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmEmerald
plasmid copy :
Michael Davidson (Addgene plasmid # 54029)
resistance markers :
5469
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA