This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-DEST42-dre4
catalog :
170440
citations: 1
Reference
Tettey T, Gao X, Shao W, Li H, Story B, Chitsazan A, et al. A Role for FACT in RNA Polymerase II Promoter-Proximal Pausing. Cell Rep. 2019;27:3770-3779.e7 pubmed publisher
product information
Catalog Number :
170440
Product Name :
pET-DEST42-dre4
article :
doi10.1016/j.celrep.2019.05.099
id28216532
pubmed_id31242411
bacterial resistance :
Ampicillin
cloning :
backbonepET-DEST42
backbone_mutation
backbone_originInvitrogen
backbone_size7440
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The expressed his-tagged protein, pET-DEST42-dre4 (deleted amino acids 1-20) was efficiently bound to CNBR beads during the affinity purification of the antibodies.
growth strain :
Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG1828, CG1828, DRE4, DRE4/dSPT16, Dmel CG1828, Dre4, SPT16, Spt16, dSPT16, dSpt16, dspt16, l(3)62Bg, l(3)dre4, spt16
genedre4
id38248
genbank_ids
mutationDeleted amino acids 1-20
namedre4
shRNA_sequence
size3366
species
7227
Drosophila melanogaster
tags
locationC terminal on backbone
tag6xHis
locationC terminal on backbone
tagV5
resistance markers :
86
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA