This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ZE2 Wnt-4
catalog :
17043
product information
Catalog Number :
17043
Product Name :
ZE2 Wnt-4
article :
| doi | |
| id | 1885 |
| pubmed_id |
bacterial resistance :
Ampicillin
cloning :
| backbone | pT7TS |
| backbone_mutation | |
| backbone_origin | |
| backbone_size | 3000 |
| promoter | |
| sequencing_primer_3 | |
| sequencing_primer_5 | |
| vector_types |
growth notes :
Insert: wnt-4 (zebrafish) cDNA. Insert size: 1.3 Kb. To make an in situ probe, linearize with EcoRV and transcribe with SP6. To make sense RNA, linearize with Xba1 and transcribe with T7. Plasmid History: Constructed by Anne Ungar on date of 6/8/93. Species of Sequence Origin: Zebrafish. Notes for Use: Note presence of R1, Sma, BamH1 sites brought in from pBSSK during construction. The 1274 nucleotide Nsi1 fragment containing the complete wnt-4 coding region was ligated into the Pst1 site of pBSSK (zf4 in pBSSK clone 5). The 5' end is near the Kpn1 site. A 1.3 Kb fragment was cut out with EcoRV and Spe1, isolated and ligated into EcoRV/Spe1 cut pT7Ts. Citation: Ungar et al., Mechanisms of Development 52 (1995)
origin :
37
pi :
|
resistance markers :
| 348 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments