XE92 XGCNF-EnR | Addgene 17029 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

XE92 XGCNF-EnR

catalog :

17029

product information

Catalog Number :

17029

Product Name :

XE92 XGCNF-EnR

article :

doi
id	1888
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

GCNF-CS2+ and pSP64T EnR

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Xenopus Expression

growth notes :

Insert: Cut XGCNF with Bgl II, XhoI, fill and cip. Gel purify the 4.3 Kb fragment (= DNA binding domain of GCNF in CS2+). Cut pSP64T EnR w/ R1 and BamHI and fill. Gel purify the 1.2 Kb fragment (= EnT domain plus 3' UTR of Beta globin). Plasmid History: Source: SP64T EnR is from Kevin (Kimelman lab), originally from Werton Lab. Notes for Use: -cut with Xba I and transcribe with SP6 for capped RNA. Ref: Song et al., Developmental Biology, 1999.

origin :

pi :

alt_names

gcnf

nr6a1

engrailed

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	NA
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG9015, 153867_at, Apa, CG9015, Dmel CG9015, EN, En, EnR, Eng, Engr, Engrailed, Engrailed/Invected, Es, V, en1, spa2, spt
gene	en
id	36240

genbank_ids

mutation

name

XGCNF-EnR

shRNA_sequence

size

species

7227

Drosophila melanogaster