pETDuet-1 TIM9,10 | Addgene 170280 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pETDuet-1 TIM9,10

catalog :

170280

citations: 2

Reference
Weinhäupl K, Wang Y, Hessel A, Brennich M, Lindorff Larsen K, Schanda P. Architecture and assembly dynamics of the essential mitochondrial chaperone complex TIM9·10·12. Structure. 2021;: pubmed publisher
Weinhäupl K, Lindau C, Hessel A, Wang Y, Schütze C, Jores T, et al. Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Cell. 2018;175:1365-1379.e25 pubmed publisher

product information

Catalog Number :

170280

Product Name :

pETDuet-1 TIM9,10

article :

doi	10.1016/j.cell.2018.10.039
id	28216505
pubmed_id	30445040

bacterial resistance :

Ampicillin

cloning :

backbone

pETDuet-1

backbone_mutation

backbone_origin

backbone_size

5700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

origin :

pi :

alt_names

Tim9

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NdeI
promoter	Tim9: via NdeI/XhoI into the MCS2; Tim10: via BamHI/HindIII into the MCS1
sequencing_primer_3
sequencing_primer_5	unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YEL020W-A
gene	TIM9
id	856693

aliases	YHR005C-A, MRS11
gene	TIM10
id	856395

genbank_ids

mutation

name

mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag)

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae