This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode
catalog :
170240
product information
Catalog Number :
170240
Product Name :
LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode
article :
doi10.1016/j.isci.2021.103149
id28216501
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backboneUnknown
backbone_mutation
backbone_origin
backbone_size9514
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterEF1a
sequencing_primer_3
sequencing_primer_55'-ttctcaagcctcagacagtgg-3'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
geneMAP2K1
id5604
genbank_ids
mutationS218D, S222D
nameMAP2K1
shRNA_sequence
size1179
species
9606
Homo sapiens
tags
plasmid copy :
Sabatini and Wood Cancer Pathways ORFs Kit (#1000000072)
resistance markers :
4273
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA