Circular 200,100 (GAPDH) | Addgene 170120 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Circular 200,100 (GAPDH)

catalog :

170120

citations: 1

Reference
Katrekar D, Yen J, Xiang Y, Saha A, Meluzzi D, Savva Y, et al. Efficient in vitro and in vivo RNA editing via recruitment of endogenous ADARs using circular guide RNAs. Nat Biotechnol. 2022;40:938-945 pubmed publisher

product information

Catalog Number :

170120

Product Name :

Circular 200,100 (GAPDH)

article :

doi	10.1038/s41587-021-01171-4
id	28216480
pubmed_id	35145312

bacterial resistance :

Kanamycin

cloning :

backbone

AAV

backbone_mutation

backbone_origin

Custom

backbone_size

6000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

AAV

growth strain :

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

origin :

pi :

sapienstags
RNAshRNA_sequence>GCCATCAGTCGCCGGTCCCAAGCCCGGATAAAAT
GGGAGGGGGCGGGAAACCGCCTAACCATGCCGACTGATG
GCAGAAAAAAAAAAAGGCCATCCACAGTCTTCTGGGTGG
CAGTGATGGCATGGACTGTGGTCATGAGTCCTTCCACGA
TACCAAAGTTGTCATGGATGACCTTGGCCAGGGGTGCCA
AGCAGTTGGTGGTGCAGGAGGCATTGCTGATGATCTTGA
GGCTGTTGTCATACTTCTCATGGTTCACACCCATGACGA
ACATGGGGGCATCAGCAGAGAAAAAAAAAAAACTGCCAT
CAGTCGGCGTGGACTGTAGAACACTGCCAATGCCGGTCC
CAAGCCCGGATAAAAGTGGAGGGTACAGTCCACGC>sizer>species

able>

9606

Homo

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	Human U6
sequencing_primer_3
sequencing_primer_5	GACTATCATATGCTTACCGT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	G3PD, GAPD, HEL-S-162eP
gene	GAPDH
id	2597

genbank_ids

mutation

name

Circular 200,100 guide

resistance markers :

4273

tags :

Unknown