XE14 Xbhh-N | Addgene 16994 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

XE14 Xbhh-N

catalog :

16994

product information

Catalog Number :

16994

Product Name :

XE14 Xbhh-N

article :

doi
id	1888
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pT7TS

backbone_mutation

backbone_origin

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Xenopus Expression

growth notes :

Expression Construct Xenopus Banded Hedgehog. (a.k.a. Xbhh; hh1) Insert: PCR of coding region, Genbank accession number U26404. Antisense RNA: For in situ we use clone XIL in Bluescript KS. Linearize with Bam H1 and transcribe with T3. Sense RNA: Linearize with Bam H1 and transcribe with T7. References: Ekker et al., Development 121, 2337-2347 (1995) Lai et al., Development 121, 2349-2360 (1995) Subclone Xbhh-N in pT7Ts. Encodes amino terminal product with autoproteolytic cleavage. Linearize with BamH1 and transcribe with T7.

origin :

pi :

alt_names

Xbhh

hh1

banded hedgehog

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	bhh, X-hh, bhh-a
gene	ihh
id	378540

genbank_ids

mutation

name

Xbhh-N

shRNA_sequence

size

species

8355

Xenopus laevis