This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
XP4 Xwnt-5A
catalog :
16987
product information
Catalog Number :
16987
Product Name :
XP4 Xwnt-5A
article :
doi
id1887
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepGem
backbone_mutation
backbone_origin
backbone_size2900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Insert: PCR clone with wnt primers. Insert size: 380 bp. Probe cut with BamH1 and transcribe with SP6. Plasmid History: Constructed by Jan Christian and Randall Moon. Note for Use: Cut with BamH1, transcribe with SP6, yielding 165 nt probe, 142 nt protected fragment. Hybridize 45 degrees C with 50,000 cpm probe (higher probe yields unacceptable background). Run 8% sequencing gel 1.5 hours.
origin :
37
pi :
alt_names
Wnt-5A
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRI
promoter
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesXwnt-5A, wnt-5a, wnt5a-a, wnt5a-b
genewnt5a.L
id378507
genbank_ids
mutation
nameXwnt-5A
shRNA_sequence
size380
species
8355
Xenopus laevis
tags
resistance markers :
348
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA