This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PARP1 FL
catalog :
169815
citations: 1
Reference
Ogden T, Yang J, Schimpl M, Easton L, Underwood E, Rawlins P, et al. Dynamics of the HD regulatory subdomain of PARP-1; substrate access and allostery in PARP activation and inhibition. Nucleic Acids Res. 2021;49:2266-2288 pubmed publisher
product information
Catalog Number :
169815
Product Name :
PARP1 FL
article :
doi10.1093/nar/gkab020
id28216430
pubmed_id33511412
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_origin
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses codon optimised full length His tagged PARP1 in bacterial cells
origin :
37
pi :
alt_names
PARP1
ADPRT
PPOL
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XbaI
promoterT7 promoter
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1
genePARP1
id142
genbank_ids
NP_001609
mutationValine 762 mutated to an alanine
namePoly[ADP-ribose] polymerase 1
shRNA_sequence
size3042
species
9606
Homo sapiens
100
Synthetic
32644
Other
Codon optimised
tags
locationN terminal on insert
tagMKHHHHHHMKQ
plasmid copy :
The original codon optimised PARP-1 gene was purchased from Qiagen, this is identical to the submitted plasmid with the exception of the V762A mutation
resistance markers :
5448
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA