pET3a-SF-LMNC-TS | Addgene 169374 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET3a-SF-LMNC-TS

catalog :

169374

citations: 1

Reference
Bertolini M, Fenzl K, Kats I, Wruck F, Tippmann F, Schmitt J, et al. Interactions between nascent proteins translated by adjacent ribosomes drive homomer assembly. Science. 2021;371:57-64 pubmed publisher

product information

Catalog Number :

169374

Product Name :

pET3a-SF-LMNC-TS

article :

doi	10.1126/science.abc7151
id	28216396
pubmed_id	33384371

bacterial resistance :

Ampicillin

cloning :

backbone

pET3a

backbone_mutation

NdeI + BamHI were used to open the MCS

backbone_origin

backbone_size

4600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

E. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

origin :

pi :

alt_names

gene: LMNA

Protein isoform: Lamin C

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	NdeI
promoter	Native pET3a promotor
sequencing_primer_3	T7 terminator rev primer
sequencing_primer_5	T7 promotor fwd primer
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1
gene	LMNA
id	4000

genbank_ids

NM_001282625

mutation

deleted amino acid 1-30 (unstructured head domain), two silent mutations in the LMNA gene (isolated from U2OS cells) at plasmid position C4960T, G5157A

name

LMNA residues 31-542

shRNA_sequence

size

1752

species

9606

Homo sapiens